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2020 Vol.65, Issue 3 Preview Page

Original Research Article

Characterization of Traits Related to Grain Shape in Korean Rice Varieties

국내 육성 벼 품종 입형 관련 특성 분석

Chang-Min Lee1
,
Keon-Mi Lee1
,
Man-Kee Baek1
,
Woo-Jae Kim1
,
Jung-Pil Suh1
,
Oh-Young Jeong2
,
Young-Chan Cho2
,
Hyun-Su Park1*
,
Suk-Man Kim3*
이 창민1
,
이 건미1
,
백 만기1
,
김 우재1
,
서 정필1
,
정 오영2
,
조 영찬2
,
박 현수1*
,
김 석만3*
1Senior Agriculture Scientist, Crop Breeding Division, National Institute of Crop Science
2Agriculture Scientist, Crop Breeding Division, National Institute of Crop Science
3Postdoctoral Research Fellow, Crop Breeding Division, National Institute of Crop Science
1농촌진흥청 국립식량과학원 작물육종과 농업연구관
2농촌진흥청 국립식량과학원 작물육종과 농업연구사
3농촌진흥청 국립식량과학원 작물육종과 전문연구원
* Corresponding Author
1 September 2020. pp. 199-213
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Abstract

Grain size and shape are the two important components contributing to rice yield and quality. To analyze traits related to grain-shape, a total of 272 varieties derived from japonica, japonica black and Tongil-type rice accession in Korea were evaluated in this study. The traits, grain length (GL), grain width (GW), grain thickness (GT), length to width ratio (RLW), and 1000-grain weight (TGW) were measured and replicated 10 times. Genes (GW2, GS3, qGL3, qSW5, GS5, TGW6, GW7, and GW8) related to grain-shape were validated in the accessions using specific DNA marker sets. K-mean clustering of the accession based on phenotypic data revealed three groups: group 1 was classified by GW and GT and included most of japonica type, group 2 was classified by RLW and GL reached a medium size and possessed a half spindle-shaped type, and group 3 was classified by TGW, reached a long size and possessed a semi-round shape. In validation tests using the marker sets, both gw2 and tgw6 were validated in less than 1% of the tested accessions and two allelic types, qgl3 and gw8, were only verified in Tongil-type accessions. For GW8 and GW2, any different amplicons were not amplified in any japonica or Tongil-type accessions, respectively. In order to suggest the representative grain-shape gene combinations for each ecotype, the allelic combinations were evaluated by PCR analysis. Cj1 and 2 in japonica (Cj1-7), Cj_b1 and 2 in japonica-black (Cj_b1-3), and CT3 in Tongil-type (CT1-13) turned out to be the dominant combination in each ecotype, respectively. In addition, the results revealed that introgression of four genes (gw2, gs3, qSW5, and GS5) would expand the diversity of grain shape in Korean japonica varieties. The gene combinations information could be utilized practically to understand or enhance grain shape in japonica rice breeding program.

Keywords
grain shape
grain-shape gene
grain size
Korea rice resource
rice
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 65
  • No :3
  • Pages :199-213
  • Received Date : 2020-05-23
  • Revised Date : 2020-06-29
  • Accepted Date : 2020-07-06
  • DOI :https://doi.org/10.7740/kjcs.2020.65.3.199
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