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2018 Vol.63, Issue 1 Preview Page

Original Research Article

Germination and Proteome Profile Characteristics of Wheat Seeds Treated under Different Concentrations of Abscisic Acid

Abscisic acid 농도에 따른 밀 종자의 발아와 단백질체의 발현 특성

Jae-Hyeok Jeong1
,
Dae-Wook Kim1
,
Woon-Ha Hwang1
,
Sung-Hyun An1
,
Han-Yong Jeong1
,
Hyeon-Seok Lee1
,
In-Bea Choi1
,
Kyung-Jin Choi1
,
Jong-Tak Yun1
,
Song Joong Yun2*
정 재혁1
,
김 대욱1
,
황 운하1
,
안 승현1
,
정 한용1
,
이 현석1
,
최 인배1
,
최 경진1
,
윤 종탁1
,
윤 성중2*
1Crop Production and Physiology Research Division, National Institute of Crop Science, Rural Development Administration
2Department of Crop Science & Biotechnology, Chonbuk National University
1농촌진흥청 국립식량과학원 작물재배생리과
2전북대학교 농업생명과학대학 작물생명과학과
*Corresponding Author
31 March 2018. pp. 25-34
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Abstract

This study was conducted to investigate the germination and proteome profile characteristics of wheat seeds treated under various concentrations of abscisic acid (ABA). After-ripening, the seeds of three wheat cultivars (Baegjoong, Keumkang, and Uri) showing different levels of dormancy were used. Germination index and germination rate of the cultivars was higher than 0.95% and 98%, respectively, and these were not significantly different under 0, 10, 30, and 50 μM ABA at 7 d after germination. However, the growth of the shoot and radicle was significantly inhibited at 10, 30, and 50 μM ABA compared to that at 0 μM ABA. Mean ABA content of the embryos of seeds germinated at 0 and 50 μM ABA for 7 d was 0.8 and 269.0 ngmg-1 DW, respectively. Proteins extracted from embryos germinated for 4 d were analyzed by two-dimensional gel electrophoresis, and proteins showing a difference of 1.5-fold or greater in their spot volume relative to that of 0 μM ABA were identified. The expression of four protein spots increased at 50 μM ABA and two protein spots were detected only at 50 μM ABA; these six proteins were all identified as globulin types. Conversely, the expression of three protein spots decreased at 50 μM ABA and were identified as cytosolic glutamine sysnthetase, isocitrate dehydrogenase, and S-adenosylmethionine synthetase 2. In conclusion, ABA did not inhibit the germination rate regardless of pre-harvest sprouting characteristics of the cultivars. However, the growth of the shoot and radicle was significantly inhibited by ABA, most likely through the down regulation of glutamine, methyl group donor, and polyamines biosynthesis, among others, while accompanied by globulin accumulation in the embryos.

Keywords
ABA
germination
globulin
proteome
wheat
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 63
  • No :1
  • Pages :25-34
  • Received Date : 2017-09-06
  • Revised Date : 2017-12-14
  • Accepted Date : 2017-12-23
  • DOI :https://doi.org/10.7740/kjcs.2018.63.1.025
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