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2020 Vol.65, Issue 4 Preview Page
Development of Disease-resistant Japonica Rice Varieties and Effects of Pyramiding Resistance Genes

내병성 자포니카 벼 계통 육성과 저항성 유전자 집적효과

Woo-Jae Kim1*
,
Man-Kee Baek1
,
Hyeon-Su Park1
,
Geon-Mi Lee1
,
Chang-Min Lee1
,
Seok-Man Kim2
,
Young-Chan Cho3
,
Jeong-Phil Seo3
,
O-Young Jeong3
김 우재1*
,
백 만기1
,
박 현수1
,
이 건미1
,
이 창민1
,
김 석만2
,
조 영찬3
,
서 정필3
,
정 오영3
1Junior Researcher, Crop breeding Division, National Institute of Crop Science, Rural Development Administration
2Postdoctoral Researcher, Crop breeding Division, National Institute of Crop Science, Rural Development Administration
3Senior Researcher, Crop breeding Division, National Institute of Crop Science, Rural Development Administration
1농촌진흥청 국립식량과학원 작물육종과 농업연구사
2농촌진흥청 국립식량과학원 작물육종과 전문연구원
3농촌진흥청 국립식량과학원 작물육종과 농업연구관
*Corresponding Author
1 December 2020. pp. 314-326
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Abstract

This study was carried out to develop a resistant variety against the K3a race of bacterial blight, Xanthomonas oryzae pv. oryzae, through expansion and pyramiding of resistance genes. To develop an elite bacterial blight-resistant cultivar, the breeding process and bacterial blight resistance reactions in advanced backcross lines (ABLs) were analyzed. ABLs21 which contain Xa3 and Xa21, were developed by double backcrossing japonica cultivar Hwanggeumnuri, which has bacterial blight resistant Xa3 gene, and indica variety IRBB21, which havs Xa21 gene, followed by disease resistance bioassay and marker-assisted selection. The resistance genes of ABLs21 were amplified by PCR with the molecular markers 9643.T4 (Xa3) and U1/I1 (Xa21). Hwanggeumnuri and IRBB3 showed resistance reactions against K1, K2, and K3 races, and a susceptible reaction against K3a, K4, and K5 races. IRBB21 showed resistance reactions against K2, K3, K3a, K4 and K5 races, and a susceptible reaction against K1 race. Hwanggeumnuri showed susceptible reactions at the seedling, tillering and adult stages (all stages), whereas ABL21-1 showed moderate resistance at the tillering stage. ABL21-1 showed stable resistance against 18 isolates of K3a race, and the lesion length was shorter than that of the donor parents. In cluster analysis, the HB4032 isolate showed the highest pathogenicity among the 18 isolates. The molecular marker polymorphisms and average substituted chromosome segment lengths of ABLs21 were 63.2 % and 86.1 cM, respectively. Insertion of the donor chromosomal segments occurred in the predicted region of the Xa21 gene of ABLs21.

Keywords
bacterial blight
chromosome segments
gene pyramiding
multi-resistant
rice
References
1
Ahn, S. B. 1973. Studies on the varietal difference in the physiology of ripening in rice with special reference to raising the percentage of ripened grains. J. Korean Soc. Crop Sci. 14 : 1-40.
2
Ali, F., D. L. E. Waters, B. Ovenden, P. Bundock, C. A. Raymond, and T. J. Rose. 2019. Australian rice varieties vary in grain yield response to heat stress during reproductive and grain filling stages. J. Agro Crop Sci. 205 : 179-187. 10.1111/jac.12312
3
Ashida, K., E. Araki, S. Iida, and T. Yasui. 2010. Flour properties of milky-white rice mutants in relation to specific loaf volume of rice bread. Food Sci. Technol. Res. 16(4) : 305-312. 10.3136/fstr.16.305
4
Ashida, K., S. Iida, and T. Yasui. 2009. Morphological, physical, and chemical properties of grain and flour from chalky rice mutants. Cereal Chem. 86(2) : 225-231. 10.1094/CCHEM-86-2-0225
5
Bae, H. K., J. D. Hwang, J. H. Seo, and S. Y. Kim. 2018. Optimal transplanting time for 'Saemimyeon' production in Youngnam province. Korean J. Crop Sci. 63(3) : 205-209.
6
Choi, W. Y., J. K. Nam, S. S. Kim, J. H. Lee, J. H. Lee, J. H. Kim, H. K. Park, N. H. Back, M. G. Choi, C. K. Kim, and K. Y. Jung. 2005. Optimum transplanting date for production quality rice in Honam plain area. Korean J. Crop Sci. 50(6) : 435-441.
7
Han, C. M., J. H. Shin, S. K. Kim, T. Y. Kwon, and J. S. Kim. 2018. Influence of different transplanting dates on amylopectin branch-chain-length and pasting properties of rice flour varieties. Korean J. Crop Sci. 63(3) : 210-218.
8
Ichikawa, T. 2017. Cooking properties of rice flour. Japan. J. Cookery Sci. 50(6) : 280-282.
9
Ishii, T. 2017. Perspectives on feed rice breeding in Japan. Grassland Sci. 63(1) : 29-33.
10
Japan Rice Flour Association (JRFA). 2020. The outlook of rice flour consumption in Japan. http ://www.komeko.org (Accessed Feb. 20, 2020).
11
Jeon, Y. H. and B. I. Ahn. 2016. Influence of family structure on the food consumption pattern. Rural Economic. 39(4) : 73-95.
12
Jeung, J. U. and Y. S. Shin. 2011. Evaluations on the Namil(SA)- flo1, a floury japonica rice line, for dry milling process to produce rice flour. Korean J. Crop Sci. 56(1) : 57-63. 10.7740/kjcs.2011.56.1.057
13
Kang, H. G., S. H. Park, M. Matsuoka, and G. H. An. 2005. White-core endosperm floury endosperm-4 in rice is generated by knockout mutations in the C4-type pyruvate orthophosphate dikinase gene(OsPPDKB). The Plant J. 42 : 901-911. 10.1111/j.1365-313X.2005.02423.x15941402
14
Kang, S. G., J. Y. Shon, H. S. Kim, S. J. Kim, J. S. Choi, J. H. Park, Y. H. Yoon, J. M. Sim, and W. H. Yang. 2018. Analysis of genetic variation in pre-harvest sprouting at different cumulative temperature after heading of rice. Korean J. Crop Sci. 63(1) : 8-17.
15
Kim, H. Y., T. Horie, H. Nakagawa, and K. Wada. 1996. Effects of elevated CO2 concentration and high temperature on growth and yield of rice. Jpn. J. Crop Sci. 65(4) : 644-651. 10.1626/jcs.65.644
16
Kim, S. J., J. G. Won, D. J. Ahn, S. D. Park, and C. D. Choi. 2008. Influence of viviparous germination on quality and yield in rice. Korean J. Crop Sci. 53(S) : 15-18.
17
Kwak, J. E., M. R. Yoon, J. S. Lee, J. H. Lee, S. H. Ko, T. H. Tai, and Y. J. Won. 2017. Morphological and starch characteristics of the Japonica rice mutant variety Seolgaeng for dry-milled flour. Food Sci. Biotechnol. 26(1) : 43-48. 10.1007/s10068-017-0006-530263508PMC6049481
18
Lee, D. J., J. H. Kim, and K. S. Kim. 2014. Spatiotemporal assessment of the late marginal heading date of rice using climate normal data in Korea. Korean J. Agric. Forest Meteorol. 16(4) : 316-326. 10.5532/KJAFM.2014.16.4.316
19
Lee, K. J., D. N. Nguyen, D. H. Choi, H. Y. Ban, and B. W. Lee. 2015a. Effects of elevated air temperature on yield and yield components of rice. Korean J. Agric. Forest Meteorol. 17(2) : 156-164. 10.5532/KJAFM.2015.17.2.156
20
Lee, K. J., D. J. Kim, H. Y. Ban, and B. W. Lee. 2015b. Genotype differences in yield and yield-related elements of rice under elevated air temperature conditions. Korean J. Agric. Forest Meteorol. 17(4) : 306-316. 10.5532/KJAFM.2015.17.4.306
21
Ministry of Agriculture, Food and Rural Affairs (MAFRA). 2019. Agriculture, food, and rural affairs statistics yearbook. Sejong, Korea. p. 375.
22
Ministry of Agriculture, Forestry and Fisheries (MAFF). 2017. The rice flour application-specific standards of Japan. Tokyo, Japan.
23
Mo, Y. J. and J. U. Jeung. 2020. The use of floury endosperm mutants to develop rice cultivars suitable for dry milling. Plant Biotechnol. Rep. (published online). 10.1007/s11816-020-00604-x
24
Mo, Y. J., J. U. Jeung, Y. S. Shin, C. S. Park, K H. Kang, and B. K. Kim. 2013. Agronomic and genetic analysis of Suweon 542, a rice floury mutant line suitable for dry milling. Rice 6 : 37. 10.1186/1939-8433-6-3724321450PMC4883716
25
Ohno, T., M. Tomatsu, K. Toeda, and N. Ohisa. 2007. Gelatinization properties of aged rice and improvement of rice texture by external layer removal. Food Sci. Technol. Res. 13(4) : 301-304. 10.3136/fstr.13.301
26
Ohno, T. and N. Ohisa. 2005. Studies on textural and chemical changes in aged rice grains. Food Sci. Technol. Res. 11(4) : 385-389. 10.3136/fstr.11.385
27
Ohta, H., M. Yamaguchi A. Fukushima, R. Kaji, N. Tsuda, K. Nakagomi, T. Kataoka, T. Endo, N. Yokogami, and Y. Tamura. 2015. "Yumefuwari", a new rice cultivar for rice flour bread. Bull. Tohoku Agric. Res. Cent. 117 : 15-27.
28
Park, J. S. and H. D. Kim. 2009. Viviparous germination characteristics of rice varieties adaptable to central region of Korea. Korean J. Crop Sci. 54(3) : 241-248.
29
Peng, S., J. Huang, J. E. Sheedy, R. C. Laza, R. M. Visperas, X. Zhong, G. S. Centeno, G. S. Khush, and K. G. Cassman. 2004. Rice yields decline with higher night temperature from global warming. Nat'l Acad. of Sci. of the USA 101(27) : 9971-9975. 10.1073/pnas.040372010115226500PMC454199
30
Sato, H., T. Kataoka, Y. Tamura, K. Tamura, M. Sakai, R. Kaji, M. Okamoto, M. Nishimura, T. Yagi, R. Mizobuchi, H. Hirabayashi, H. Yamashita, S. Fukaura, H. Nishiyama, H. Motomura, T. Takita, and K. Saito. 2017. "Mizhochikara" a multipurpose high-yielding rice variety adopted in warm regions of Japan that is suitable for rice bread, forage grain and Shochu brewing material. Kyushu Okinawa Agric. Res. Cent. Rep. 66 : 47-63.
31
Satoh, H. and T. Omura. 1981. New endosperm mutations induced by chemical mutagens in rice. Japan J. Breed. 31(3) : 316-326. 10.1270/jsbbs1951.31.316
32
She, K. C., H. Kusano, K. Koizumi, H. Yamakawa, M. Hakata, T. Imamura, M. Fukuda, N. Naito, Y. Tsurumaki, M. Yaeshima, T. Tsuge, K. Matsumoto, M. Kudoh, E. Itoh, S. Kikuchi, N. Kishimoto, J. Yazaki, T. Ando, M. Yano, T. Aoyama, T. Sasaki, H. Satoh, and H. Shimada. 2010. A novel factor FLOURY ENDOSPERM2 is involved in regulation of rice grain size and starch quality. The Plant Cell. 22 : 3280-3294. 10.1105/tpc.109.07082120889913PMC2990130
33
Suh, K. H. and Y. W. Kim. 1994. Varietal difference in viviparous germination at different days after heading and temperature conditions in rice. Korean J. Crop Sci. 39(2) : 187-192.
34
Takahashi, N. 1980. Effect of environmental factors during seed formation on pre-harvest sprouting. Cereal Res. Communications. 8(1) : 175-183.
35
Won, Y. J., E. K. Ahn, E. G. Jeong, J. K. Chang, J. H. Lee, K. H. Jung, U. J. Hyun, Y. C. Cho, S. K. Oh, M. R. Yoon, B. K. Kim, and B. J. Kim. 2019. An opaque endosperm rice cultivar, 'Hangaru', suitable for exclusive dry-milling rice flour production. Korean J. Breed. Sci. 51(2) : 134-139. 10.9787/KJBS.2019.51.2.134
36
Yang, W. H. J. W. Park, J. S. Choi, S. G. Kang, and S. J. Kim. 2019. Yield characteristics and related agronomic traits affected by the transplanting date in early maturing varieties of rice in the central plain area of Korea. Korean J. Crop Sci. 64(3) : 165-175. 10.2135/cropsci2018.05.0349
37
Yang, W. H., S. G. Kang, S. J. Kim, J. S. Choi, J. W. Park, and Y. H. Yoon. 2018. Changes of heading date and yielding characterstics as affected by transplanting date in the mid-maturing rice cultivar 'Haiami' in central plain area of Korea. J. Korean Soc. Int. Agric. 30(1) : 51-57. 10.12719/KSIA.2018.30.1.51
38
Yoshii, Y., N. Homma, and R. Akaishi. 2011. Development of rice processing technology in Niigata Prefecture. Japanese Soc. Food Sci. and Tech. 58(5) : 187-195. 10.3136/nskkk.58.187
Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 65
  • No :4
  • Pages :314-326
  • Received Date : 2020-05-19
  • Revised Date : 2020-09-04
  • Accepted Date : 2020-09-27
  • DOI :https://doi.org/10.7740/kjcs.2020.65.4.314
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