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2020 Vol.65, Issue 3 Preview Page

Original Research Article

Selection of Salt-Tolerant Silage Rice Through in vitro Screening and Saltol QTL Analysis

기내 선발과 Saltol QTL 분석을 통한 내염성 증진 사료용 벼 선발

Chuloh Cho1
,
Kyung Hwa Kim2
,
Eok-Keun Ahn3
,
Hyangmi Park4
,
Man-Soo Choi5
,
Jaebuhm Chun5
,
Mi-Suk Seo5
,
Mina Jin6
,
Dool-Yi Kim5*
조 철오1
,
김 경화2
,
안 억근3
,
박 향미4
,
최 만수5
,
전 재범5
,
서 미숙5
,
진 민아6
,
김 둘이5*
1PostDoctor, Crop Foundation Research Division, National Institute of Crop Science, RDA
2Staff, Crop Foundation Research Division, National Institute of Crop Science, RDA
3Researcher, Department of Central Area Crop Science, National Institute of Crop Science, RDA
4Senior Researcher, Department of Central Area Crop Science, National Institute of Crop Science, RDA
5Researcher, Crop Foundation Research Division, National Institute of Crop Science, RDA
6Senior Researcher, Crop Foundation Research Division, National Institute of Crop Science, RDA
1농촌진흥청 국립식량과학원 작물기초기반과 전문연구원
2농촌진흥청 국립식량과학원 작물기초기반과 연구원
3농촌진흥청 국립식량과학원 중부작물부 농업연구사
4농촌진흥청 국립식량과학원 중부작물부 농업연구관
5농촌진흥청 국립식량과학원 작물기초기반과 농업연구사
6농촌진흥청 국립식량과학원 작물기초기반과 농업연구관
* Corresponding Author
1 September 2020. pp. 214-221
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Abstract

Salinity is one of the major abiotic stressors that inhibits the growth, yield, and productivity of crop plants. Therefore, it is necessary to develop crops with increased salt tolerance for cultivation in saline soils such as is found in reclaimed land. The objective of this study was to develop a salt-tolerant silage rice line that grows on reclaimed land. In order to develop this salt-tolerant silage rice, we transferred Saltol, a major QTL associated with salt tolerance, from IR64-Saltol, a salt-tolerant indica variety, into Mogyang, a susceptible elite japonica variety. To determine the effect of salt stress, Mogyang and IR64-Saltol cultivars were grown on a medium containing various concentrations of NaCl in in vitro conditions. Shoot length was found to decrease with increasing salt concentrations, and root growth was almost arrested at NaCl concentrations over 50 mM in the Mogyang cultivar. Based on these preliminary results, we screened five salt-tolerant lines showing superior growth under salt stress conditions. Polymerase chain reaction and sequencing results showed that the introgression types of Saltol QTL were derived from the IR64-Saltol cultivar in almost all selected lines. Based on the observed growth and physiological characteristics, the new Saltol introgression lines showed higher salt tolerance compared to the Mogyang parental cultivar. The salt-tolerant lines identified in this study could be used as a genetic resource to improve rice salt tolerance.

Keywords
mogyang
reclaimed land
Saltol QTL
salt-tolerant
silage rice
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 65
  • No :3
  • Pages :214-221
  • Received Date : 2020-05-18
  • Revised Date : 2020-07-02
  • Accepted Date : 2020-07-29
  • DOI :https://doi.org/10.7740/kjcs.2020.65.3.214
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