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2018 Vol.63, Issue 4 Preview Page

Original Research Article

Physiological and Genetic Responses of Salt-stressed Tunisian Durum (Triticum turgidum ssp. durum) Cultivars
Sang Heon Kim1
,
Dae Yeon Kim2
,
Inés Yacoubi3
,
Yong Weon Seo45*
,
,
,
1Ph.D. Candidate, Department of Biosystems and Biotechnology, Korea University
2Research Professor, Division of Biotechnology, Korea University
3Associate Professor, Centre of Biotechnology of Sfax
4Professor, Department of Biosystems and Biotechnology, Korea University
5Professor, Division of Biotechnology, Korea University
*Corresponding Author
31 December 2018. pp. 314-321
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Abstract

Durum (Triticum turgidum L. ssp. durum) is a major crop species cultivated for human consumption worldwide. In Tunisia, salt stress is one of the main problems that limit crop production. ‘Mahmoudi’ was selected as the most salt-sensitive out of 11 Tunisian durum cultivars. Using the salt-tolerant cultivar ‘Om Rabia’, resistant and susceptible cultivars were evaluated to compare genetic responses under salt stress. At the fully expanded third leaf stage, salt stress was applied by submerging the pots in 500 mM NaCl for 5 min every day for saline water irrigation in the greenhouse. The treatment was applied for 1 week and salt stress tolerance was determined by changes of growth parameters to the control condition. The salt tolerance trait index and salt tolerance index were calculated and used as selection criteria. The expression levels of TdHKT1;4, TdHKT1;5, and TdSOS1 were examined using qPCR. For further evaluation of physiological responses, salt stress (150 mM NaCl) was additionally applied for 48 h at the fully expanded third-leaf stage. Increased expression of the genes responsible for salt tolerance and proline content in tolerant durum can be used to broaden genetic diversity and provide genetic resources for the durum breeding program.

Keywords
durum
growth parameter
proline
salt tolerance
Tunisian durum
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 63
  • No :4
  • Pages :314-321
  • Received Date : 2018-09-08
  • Revised Date : 2018-10-01
  • Accepted Date : 2018-10-02
  • DOI :https://doi.org/10.7740/kjcs.2018.63.4.314
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