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2018 Vol.63, Issue 2 Preview Page
June 2018. pp. 158-163
Abstract
Perilla (Perilla frutescens var.frutescens) is an annual plant of the Lamiaceae family, mainly grown for obtaining oil by press extraction after roasting the seeds. Oil yield is one of its important traits, but evaluating this yield is time-consuming, requires many seeds, and is hard to adjust to pedigrees in a breeding field. The objective of this study was to develop a method for selecting high-oil-yield lines in a breeding population without oil extraction. Twenty-three perilla cultivars were used for evaluating the oil yield and seed traits such as seed hardness, seed coat thickness, seed coat proportion and crude fat. After evaluation of the seed traits of 23 perilla cultivars, the ranges of oil yields, seed hardness, seed coat thickness, seed coat proportion, 100-seed weight, and crude fat were 24.68-38.75%, 157-1166 gf, 24-399 ㎛, 15.4-41.5%, 2.79-6.69 g, and 33.0-47.8%, respectively. In an analysis of correlation coefficients, the oil yield negatively correlated with seed length, seed width, the proportion of seed coat, seed hardness, and 1000-seed weight, but positively correlated with crude fat content. It was observed that as the seed coat proportion increased, the seed coat thickness, hardness, and 1000-seed weight also increased. Multiple linear regression (MLR) was employed to find major variables affecting the oil yield. Among the variables, traits crude fat content and seed coat proportion were assumed to be indirect parameters for estimating the potential oil yield, with respect to a significant positive correlation with the observed oil yield (R2 = 0.791). Using these two parameters, an equation was derived to predict the oil yield. The results of this study show that various seed traits in 23 perilla cultivars positively or negatively correlated with the oil yield. In particular, crude fat and the seed coat proportion can be used for predicting the oil yield with the newly developed equation, and this approach will improve the efficiency of selecting prominent lines for the oil yield.


References
  1. Aremu, A. K. and C. A. Ogunlade. 2016. Influence of moisture content and seed dimensions on mechanical oil expression from African oil bean (Pentaclethra Macrophylla benth) seed. Journal of Biosystems Engineering, 41(3), 193-200.https://doi.org/10.5307/JBE.2016.41.3.193
  2. Asif, M. 2011. Health effects of omega-3, 6, 9 fatty acids : Perilla frutescens is a good example of plant oils. Oriental Pharmacy and Experimental Medicine, 11, 51-59. https://doi.org/10.1007/s13596-011-0002-x
  3. Bandara, D. M. S. P., C. A. K. Dissanayake, T. M. R. Dissanayake, H. M. A. P. Rathanayake., and D. P. Senanayake. 2016. Performance evaluation of the screw-type oil expeller for extracting mee (Madhuca longifolia) oil. Journal of Biosystems Engineering, 41(3), 177-183https://doi.org/10.5307/JBE.2016.41.3.177
  4. Banno, N., T. Akihisa, H. Tokuda, K. Yasukawa, H. Higashihara, M. Ukiya, and H. Nishino. 2014. Triterpene acids from the leaves of Perilla frutescens and their anti-inflammatory and antitumor-promoting effects triterpene acids from the leaves of Perilla frutescens. Bioscience, Biotechnology, and Biochemistry, 8451(1), 85-90. https://doi.org/10.1271/bbb.68.85https://doi.org/10.1271/bbb.68.85
  5. Bin, Y. H., Z. R. Choe, M. S. Yang, and S. H. Kim. 1988. Effects of date and degrees of defoliation on seed yield and fatty acid composition of perilla (Perilla ocymoids L.). Korean Journal of Crop Science1, 33(2), 182-188.
  6. Choung, M. G. 2005. Comparision of major characteristics between seed perilla and vegetable perilla. Korean Journal of Crop Science, 50(S), 171-174.
  7. Eggers, R. 1985. High pressure extraction of oil seed. Journal of the American Oil Chemists’ Society, 62(8), 1222-1230.https://doi.org/10.1007/BF02541832
  8. FIS. 2017. Food Information Statistics System. http://www.atfis.or.kr
  9. Guixiang, Z., T. D. Etherton, K. R. Martin, S. G. West, P. J. Gillies, and P. M. Kris-Etherton. 2004. Human nutrition and metabolism dietary a-linolenic acid reduces inflammatory and lipid cardiovascular risk factors in hypercholesterolemic men and women. The Journal of Nutrition, 134(11), 2991-2997.https://doi.org/10.1093/jn/134.11.299115514264
  10. Ha, T. J., J. H. Lee, M. H. Lee, B. W. Lee, H. S. Kwon, C. H. Park, K. Shim, H. Kim, I. Baek, and D. S. Jang. 2012. Isolation and identification of phenolic compounds from the seeds of Perilla frutescens (L.) and their inhibitory activities against a -glucosidase and aldose reductase. Food Chemistry, 135(3), 1397-1403. https://doi.org/10.1016/j.foodchem.2012.05.104https://doi.org/10.1016/j.foodchem.2012.05.104
  11. Hamazaki, T., S. Sawazaki, M. Itomura, E. Asaoka, Y. Nagao N. Nishimura, and M. Kobayashi. 1996. The effect of docosahexaenoic acid on aggression in young adults. Journal of Clinical Investigation, 97(4), 1129-1133.https://doi.org/10.1172/JCI1185078613538PMC507162
  12. Hu, Z., W. Hua, L. Zhang, L. Deng, X. Wang, G. Liu, W. Hao, H. Wang. 2013. Seed structure characteristics to form ultrahigh oil content in rapeseed. PLOS ONE, 8(4), 2-11. https://doi.org/ 10.1371/journal.pone.0062099https://doi.org/10.1371/journal.pone.006209923637973PMC3639247
  13. Jamal, A., I. S. Fazli, S. Ahmad, M. Z. Abdin, and S. J. Yun. 2005. Effect of sulphur and nitrogen application on growth characteristics, seed and oil yields of soybean cultivars. Korean Journal of Crop Science2, 50(5), 340-345.
  14. James, M. J., R. A. Gibson, L. G. Cleland. 2000. Dietary polyunsaturated fatty acids and inflammatory mediator production. The American Journal of Clinical Nutrition, 71(S), 343-348.
  15. Kim, M., J. H. Nam, D. H. Oh, and Y. Park. 2010. Erythrocyte α -linolenic acid is associated with the risk for mild dementia in Korean elderly. Nutrition Research, 30(11), 756-761. https://doi.org/10.1016/j.nutres.2010.10.005https://doi.org/10.1016/j.nutres.2010.10.005
  16. Kim, Y. E., I. H. Kim, S. Y. Jung, S. Y, and J. S. Jo. 1996. Changes in components and sensory attribute of the oil extracted from perilla seed roasted at different roasting conditions. Journal of the Korean Society of Agricultural Chemistry and Biotechnology, 39(2), 118-122.
  17. KOSIS; Korean Statistical Information Service. 2018. Retrieved from http://kostat.go.kr
  18. Lee, A. Y., M. H. Lee, S. Lee and E. J. Cho. 2017. Alpha- linolenic acid from perilla frutescens var. japonica oil protects Aβ-induced cognitive impairment through regulation of APP Processing and Aβ degradation. Journal of Agricultural and Food Chemistry, 65(49), 10719-10729. https://doi.org/10.1021/ acs.jafc.7b03941
  19. Lee, M. H., C. S. Jung, K. W. Oh, C. B. Park, D. G. Kim, J. K. Choi, S. Y. Nam. 2011. A new perilla cultivar for edible seed “Dayu” with high oil content. Korean Journal of Breeding Science, 43(6), 616-619.
  20. Lee, S. W., C. H. Park, C. W. Kang, and S. D. Kim. 1999. Changes in oil, tannin, total sugar contents and yield after flowering in peanut. Korean Journal of Crop Science, 44(2), 159-162.
  21. Lee, Y. I., I. C. Shin, and J. S. Kim. 1993. Characteristics of induced mutant lines from gamma irridated perilla. Korean Journal of Breeding Science, 26(1), 13-18.https://doi.org/10.9787/KJBS.2018.50.1.13
  22. Li, Y., F. Beisson, M. Pollard, and J.Ohlrogge. 2006. Oil content of Arabidopsis seeds : The influence of seed anatomy, light and plant-to-plant variation. Phytochemistry, 67, 904-915. https://doi.org/10.1016/j.phytochem.2006.02.015https://doi.org/10.1016/j.phytochem.2006.02.015
  23. Li, Y., Y. Zhang, X. Sui, Y. Zhang, H. Feng, and L. Jiang. 2014. Ultrasound-assisted aqueous enzymatic extraction of oil from perilla (Perilla frutescens L.) seeds. CYTA - Journal of Food, 12(1), 16-21. https://doi.org/10.1080/19476337.2013.782070https://doi.org/10.1080/19476337.2013.782070
  24. Mantese, A. I., D. Medan, and A. J. Hall. 2006. Achene structure, development and lipid accumulation in sunflower cultivars differing in oil content at maturity. Annals of Botany, 97, 999-1010. https://doi.org/10.1093/aob/mcl046https://doi.org/10.1093/aob/mcl046
  25. Min, Y. K., and Z. U. Kim. 1992. Changes of lipid during maturation of perilla seed (Perilla frutescens). Journal of the Korean Society of Agricultural Chemistry and Biotechnology, 35(3), 139-145.
  26. Oh, K. W., M. G. Choung, S. B. Pae, C. S. Jung, K. B. Joo, Y. C. Kwon, J. T. Kim, and Y. H. Kwack. 2000. Determination of seed lipid and protein contents in perilla and peanut by Near-Infrared Reflectance Spectroscopy. Korean Journal of Crop Science, 45(5), 339-342.
  27. Oh, K. W., S. B. Pae, J. T. Kim, J. G. Gwak, Y. H. Gwak, and D. U. Kim. 1997. Genetic analysis of yield related in perilla. Korean Journal of Breeding Science, 29(1), 20-21.
  28. Park, C.B., J. K. Bang, B. K. Lee, J. K. Kim, B. H. Lee, 2000. Research activity and achievement in mutation breeding of perilla. Journal of the Korean Society of International Agriculture, 12(1), 108-115.
  29. RDA; Rural Development Administration. 2017. Oilseed Crop. Rural Development Administration.
  30. Rosenthal, A., D. L. Pyle, and K. Niranjan. 1996. Aqueous and enzymatic processes for edible oil extraction. Enzyme and Microbial Technology, 19, 402-420.https://doi.org/10.1016/S0141-0229(96)80004-F
  31. Ryu, S. N., J. I. Lee, H. S. Lee, C. B. Park, B. R. Sung, 1993. Varietal difference of oil content and omega fatty acid composition in Korea local perilla. Korean Journal of Crop Science, 38(6), 560-565.
  32. SAS Institute. 2012. Cary, NC: SAS Institute Inc.
  33. Schaefer, E. J., V. Bongard, A. S. Beiser, L. F. Stefania, S. J. Robins, R. Au, K. L. Tucker, D. J. Kyle, P. W. F. Wilson, and P.A. Wolf. 2006. Plasma phosphatidylcholine docosahexaenoic acid content and risk of dementia and Alzheimer disease. Archives of Neurology, 63, 1545-1550.https://doi.org/10.1001/archneur.63.11.154517101822
  34. Tzen, J. T. C., Y. Cao, P. Laurent, C. Ratnayake, and A. H. C. Huang. 1993. Lipids, proteins, and structure of seed oil bodies from diverse species. Plant Physiology, 101, 267-276.https://doi.org/10.1104/pp.101.1.26712231682PMC158673
  35. Yamamoto, N., M. Saitoh, A. Moriuchi, and M. Nomura. 1987. Effect of dietary a-linolenate/linoleate balance on brain lipid compositions and learning ability of rats. Journal of Lipid Research, 28, 144-151.2883248
  36. Žekonis, G., J. Žekonis, R. Šadzevičienė, G. Šimonienė, and E. Kėvelaitis. 2008. Effect of Perilla frutescens aqueous extract on free radical production by human neutrophil leukocytes. Medicina (Kaunas), 44(9), 699-705.https://doi.org/10.3390/medicina44090090
  37. Zhang, T., S. Zhao, W. Li, L. Ma, M. Ding, R. Li, and Y. Liu. 2014. High-fat diet from perilla oil induces insulin resistance despite lower serum lipids and increases hepatic fatty acid oxidation in rats. Lipids in Health and Disease, 13(15), 1-9.https://doi.org/10.1186/1476-511X-13-15
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